Use the latest version of Circos and read Circos best practices—these list recent important changes and identify sources of common problems.
If you are having trouble, post your issue to the Circos Google Group and include all files and detailed error logs. Please do not email me directly unless it is urgent—you are much more likely to receive a timely reply from the group.
Don't know what question to ask? Read Points of View: Visualizing Biological Data by Bang Wong, myself and invited authors from the Points of View series.
UNIX vs Windows
3. Configuration Files - Syntax, Colors, Fonts and Units
5. Data Files
6. Required Perl Modules
7. Runtime Parameters
9. Common Errors
10. PNG Output
11. SVG Output